Providing Quality 3D Cell Culture Systems for
Tissue Engineering and Stem Cell Culture Applications

Microbiology

New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.

Mellata M, Maddux JT, Nam T, Thomson N, Hauser H, Stevens MP, Mukhopadhyay S, Sarker S, Crabbé A, Nickerson CA, Santander J, Curtiss R 3rd. PLoS One. 2012;7(1):e29481. [Abstract] [Full Text] [Full Text pdf]

Human norovirus infection of caco-2 cells grown as a three-dimensional tissue structure.

Straub TM, Bartholomew RA, Valdez CO, Valentine NB, Dohnalkova A, Ozanich RM, Bruckner-Lea CJ, Call DR. J Water Health. 2011 Jun;9(2):225-40. [Abstract] [Full Text] [Full Text pdf]

Responses of haloarchaea to simulated microgravity

Dornmayr-Pfaffenhuemer M, Legat A, Schwimbersky K, Fendrihan S, Stan-Lotter H. Astrobiology; 11(3):199-205. 2011 [Abstract]

Effects of low-shear modeled microgravity on the characterization of recombinant β-D-glucuronidase expressed in Pichia pastoris.

Qi F, Dai D, Liu Y, Kaleem I, Li C Appl Biochem Biotechnol.; 163(1):162-7, 2011[Abstract]

Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions.

Barrila J, Radtke AL, Crabbé A, Sarker SF, Herbst-Kralovetz MM, Ott CM, Nickerson CA. Nat Rev Microbiol. 2010 Nov;8(11):791-801 [Abstract]

Maintenance of antimicrobial susceptibility of Acinetobacter baumannii in modeled microgravity.

Hensley DM.Clin Lab Sci. 23(2):84-8, 2010 [Abstract]

Analysis of Interactions of Salmonella type three secretion mutants with 3-D intestinal epithelial cells.

Radtke AL, Wilson JW, Sarker S, Nickerson CA. PLoS One 29;5:e15750, 2010 [Abstract] [Full Text] [Full Text pdf]

Response of Pseudomonas aeruginosa PAO1 to low shear modeled microgravity involves AlgU regulation.

Crabbe A, Pycke B, Van Houdt R, Monsieurs P, Nickerson C, Leys N, Cornelis P. Environ Microbiol 12:1545- 1564, 2010 [Abstract]

Bacillus thuringiensis conjugation in simulated microgravity.

Beuls E, Van Houdt R, Leys Y, Dijkstra C Larkin O, Mahillon J. Astrobiology. 9: 797-805, 2009 [Abstract]

Use of the rotating wall vessel technology to study the effect of shear stress on growth behaviour of Pseudomonas aeruginosa PA01.

Crabbé A, De Boever P, Van Houdt R, Moors H, Mergeay M, Cornelis P. Environ Microbiol 10: 2098-2110, 2008 [Abstract]

Detection of Epithelial-Cell Injury, and Quantification of Infection, in the HCT-8 Organoid Model of Cryptosporidiosis.

Warren CA, Destura, RV, Sevilleja JEAD, Barroso LF, Carvalho H, Barrett LJ, O’Brien, AD, Guerrant RL.  J Infect Dis 198: 143-149, 2008 [Abstract] [Full Text] [Full Text pdf]

Media Ion Composition Controls Regulatory and Virulence Response of Salmonella in Spaceflight. 

Wilson JW ,Ott CM, Quick L, Davis R, Zu Bentrup KH, Crabbe´ A, Richter E, Sarker S, Barrila J, Porwollik S, Cheng P, McClelland M, Tsaprailis G, Radabaugh T, Hunt A, Shah M, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, deBaca AC, Narayan S, Benjamin J, Goulart C, Rupert M, Catella L, Schurr MJ, Buchanan K, Morici L, McCracken J, Porter MD, Pierson DL, Smith SM, Mergeay M, Leys N, Stefanyshyn-Piper HM, Gorie D, Nickerson CA. PLos ONE 3: e3923, 2008 [Abstract] [Full Text] [Full Text pdf]

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq.

Wilson JW, Ott CM, Zu Bentrup KH, Ramamurthy R, Quick L, Porwollik S, Cheng P, McClelland M, Tsaprailis G, Radabaugh T, Hunt A, Fernandez D, Richter E, Shah WM, Kilcoyne M, Joshi L, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, Goulart C, Rupert M, Stodieck L, Stafford P, Catella L, Schurr MJ, Buchanan K, Morici L, McCracken J, Allen P, Baker-Colman C, Hammond T, Vogel J, Nelson R, Pierson DL, Stefanyshyn-Piper HM, Nickerson CA. Proc Natl Acad Sci USA 104: 16299-16304, 2007 [Abstract] [Full Text] [Full Text pdf]

Studying host-pathogen interactions in 3-D: organotypic models for infectious disease and drug development.

Nickerson CA, Richter EG, Ott CM; J Neuroimmune Pharmacol. 2:26-31, 2007 [Abstract]

In vitro Cell Culture Infectivity Assay for Human Noroviruses.

Straub TM, Honer zu Bentrup K, Orosz-Coghlan, P, Dohnalkova A, Mayer BK, Bartholomew RA, Valdez CO, Bruckner-Lea C, Gerba CP, Abbaszadegan M, Nickerson CA,  Emerging Infectious Diseases 13: 396-403, 2007 [Abstract] [Full Text] [Full Text pdf]

Characterization of Escherichia coli MG1655 grown in a low shear modeled microgravity environment.

Tucker DL, Ott CM, Huff, S, Fofanov V, Willson RC, Fox GE. BMC Microbiology 7: 15, 2007 [Abstract] [Full Text] [Full Text pdf]

A Novel Quantitative Biosystem to Model Physiological Fluid Shear Stress on Cells.

Nauman EA, Ott CM, Sander E, Tucker DL, Pierson D, Wilson JW, Nickerson CA., Appl Environ Microbiol 73:699-705, 2006 [Abstract] [Full Text] [Full Text pdf]

Novel three-dimensional organoid model for evaluation of the interaction of uropathologenic Escherichia coli with terminally differentiated human urothelial cells.

Smith YC, Grande KK, Rasmussen SB, O’Brien AD.;  Infect. Immun. 74: 750-757, 2006 [Abstract] [Full Text] [Full Text pdf]

Escherichia coli biofilms formed under low-shear modeled microgravity in a ground-based system.

Lynch SV, Mukundakrishnan K, Benoit MR, Ayyaswamy PS, Matin A;. Appl. Environ. Microbiol. 72: 7701-7710, 2006 [Abstract] [Full Text] [Full Text pdf]

Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis.

Honer Zu Bentrup K, Ramamurthy R, Ott CM, Emami K, Nelman-Gonzalez M, Wilson JW, Richter EG, Goodwin TJ, Alexander JS, Pierson DL, Pellis N, Buchanan KL, Nickerson CA. Microbes Infect. 8: 1813-182, 2006. [Abstract]

A three-dimensional tissue culture model for the study of attach and efface lesion formation by enteropathic and enterohaemorrhagic Escherichia coli.

CCarvalho HM, Teel LD, Goping G, O’Brien AD: ell Microbiol 7: 1771-1781, 2005. [Abstract]

A549 Lung Epithelial Cells Grown as Three-Dimensional Aggregates: Alternative Tissue Culture Model for Pseudomonas Aeruginosa Pathogenesis.

Carterson AJ, Honer zu Bentrup K, Ott CM, Clarke MS, Pierson DL, Vanderburg CR, Buchanan KL, Nickerson CA, Schurr MJ:Infect Immun 73: 1129-1140, 2005. [Abstract] [Full Text] [Full Text pdf]

Invasion of Human Tissue Ex Vivo by Borrelia:

Duray P, Yin S, Ito Y, Bezrukov L, Cox C, Cho M, Fitzgerald W: Journal of infectious Diseases 191:1747-1754 2005 [Abstract] [Full Text] [Full Text pdf]

A Potential Cause for Kidney Stone Formation During Space Flights: Enhanced Growth of Nanobacteria in Microgravity.

Ciftcioglu N, Haddad RS, Golden DC, Morrison DR, McKay DS: Kidney Int. 67: 483- 491, 2005. [Abstract]

Three-Dimensional Growth of Extravillous Cytotrophoblasts Promotes Differentiation and Invasion.

LaMarca H.L, Ott C.M, Höner zu Bentrup K, LeBlanc C.L, Pierson D.L, Nelson A.B, Scandurro A.B, Whitley G.St.J, Nickerson C.A, and C.A.Morris: Placenta 26::709-720, 2005 [Abstract]

Role and Regulation of Sigma S in General Resistance Conferred by Lowshear Simulated Microgravity in Escherichia Coli.

Lynch SV, Brodie EL, Matin A: J Bacteriol 186: 8207-8212, 2004. [Abstract] [Full Text] [Full Text pdf]

Microbial Responses to Microgravity and Other Low-Shear Environments.

Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, Pierson DL:Microbiol Mol Biol Rev Jun; 68: 345-61, 2004. [Abstract] [Full Text] [Full Text pdf]

A New Dimension in Modeling Infectious Disease.

Nickerson CA, Ott CM: ASM News 70: 169-175, 2004. [Full Text pdf]

Growth and Membrane Polarization in Pseudomonas aeruginosa UG2 Grown in Randomized Microgravity in a High Aspect Ratio Vessel.

England LS, Gorzelak M, Trevors JT: Biochimica et Biophysica Acta 1624: 76- 81, 2003. [Abstract]

Low-Shear Modeled Microgravity: A Global Environmental Regulatory Signal Affecting Bacterial Gene Expression

Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, LeBlanc CL,et al.: Physiology, and Pathogenesis. Journal of Microbiological Methods 54:1-11, 2003. [Abstract]

Saccharomyces cerevisiae Gene Expression Changes During Rotating Wall Vessel Suspension Culture

Johanson K, Allen PL, Lewis F, Cubano LA, Hyman LE, Hammond TG: J Appl Physiol. 2171-2180, 2002. [Abstract] [Full Text] [Full Text pdf]

Low-Shear Modeled Microgravity Alters the Salmonella Enterica Serovar Typhimurium Stress Response in an RpoSindependent Manner.

Applied and Environmental Microbiology 68:5408-5416, 2002. [Abstract] [Full Text] [Full Text pdf]

Microarray Analysis Identifies Salmonella Genes Belonging to the Low-Shear Modeled Microgravity Regulon

Wilson JW, Ramamurthy R, Porwollik S, McClelland M, Hammond T, Allen P, Ott CM, Pierson DL, Nickerson CA:  Proc. Natl. Acad. Sci. USA 99: 13807-13812, 2002  [Abstract] [Full Text] [Full Text pdf]

Secondary Metabolism in Simulated Microgravity

Demain AL and Fang A: Chem Rec 1: 333-346, 2001. [Abstract]

Shear Stress Enhances Microcin B17 Production in a Rotating Wall Bioreactor, But Ethanol Does Not.

Gao Q, Fang A, Pierson DL, Mishra SK, Demain AL: Appl Microbiol Biotechnol 56: 384-387, 2001. [Abstract]

Three-Dimensional Tissue Assemblies: Novel Models for the Study of Salmonella Enterica Serovar Typhimurium Pathogenesis.

Nickerson CA, Goodwin TJ, Terlonge J, Ott CM, Buchanan KL, Uicker WC, Emami K, LeBlanc C, Ramamurthy R, Clarke MS, Vanderburg CR, Hammond T, Pierson DL: Infect Immun 69: 7106-7120, 2001. [Abstract] [Full Text] [Full Text pdf]

Microgravity as a Novel Environmental Signal Affecting Microbial Virulence.

Nickerson CA, Ott M, Mister SJ, Morrow BJ, Burns-Keliher L, Pierson DL: Infect. Immun. 68: 3147-3150, 2000. [Abstract] [Full Text] [Full Text pdf]

Relief from Glucose Interference in Microcin B17 Biosynthesis by Growth in a Rotating-Wall Bioreactor.

Fang A, Pierson DL, Mishra SK, Demain AL: Lett Appl Microbiol 31: 39-41, 2000. [Abstract]

Effect of Simulated Microgravity and Shear Stress on Microcin B17 Production by Escherichia Coli and on its Excretion into the Medium.

Fang A, Pierson DL, Koenig DW, Mishra SK, and Demain AL: Appl Environ Microbiol 63: 4090-4092, 1997. [Abstract] [Full Text] [Full Text pdf]

Gramicidin S Production by Bacillus Brevis in Simulated Microgravity.

Fang A., Pierson DL, Mishra SK, Koenig DW and Demain AL: Curr Microbiol 34: 199-204, 1997. [Abstract]

Secondary Metabolism in Simulated Microgravity: B-Lactam Production by Streptomyces Clavuligerus.
Fang A, Pierson DL, Mishra SK, Koenig DW and Demain AL: J Ind Microbiol Biotechnol 18: 22-25, 1997 [Abstract]
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